| Tissue Specificity | Expressed in several tissues. Expressed in macrophages.expression is increased in macrophages from patients with Tangier disease. |
| Post Translational Modifications | Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum. |
| Function | Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP. The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol. |
| Protein Name | Atp-Binding Cassette Sub-Family G Member 1Atp-Binding Cassette Transporter 8White Protein Homolog |
| Database Links | Reactome: R-HSA-1369062Reactome: R-HSA-8964058Reactome: R-HSA-9029569 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneCell MembranePredominantly Localized In The Intracellular Compartments Mainly Associated With The Endoplasmic Reticulum (Er) And Golgi Membranes |
| Alternative CLIA Names | Atp-Binding Cassette Sub-Family G Member 1 CLIA kitAtp-Binding Cassette Transporter 8 CLIA kitWhite Protein Homolog CLIA kitABCG1 CLIA kitABC8 CLIA kitWHT1 CLIA kit |
| Specificity | This kit recognizes Human ABCG1 in samples. No significant cross-reactivity or interference between Human ABCG1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-101 | 94 |
| EDTA plasma(n=8) | 87-101 | 93 |
| Cell culture media(n=8) | 85-98 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 98.36 | 258.81 | 673.22 | 101.87 | 238.81 | 618.16 |
| Standard deviation | 8.56 | 25.91 | 50.76 | 12.47 | 27.10 | 43.46 |
| CV (%) | 8.70 | 10.01 | 7.54 | 12.24 | 11.35 | 7.03 |
