Human ABCG1 (ATP-binding cassette sub-family G member 1) CLIA Kit for Quantitative Detection

Name: Human ABCG1 (ATP-binding cassette sub-family G member 1) CLIA Kit for Quantitative Detection
SKU: STJC0000299
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJC0000299

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General Information

Short Description	This human ABCG1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	18.75pg/mL
Detection Limit	31.25~2000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Target Information

Gene Symbol	ABCG1
Gene ID	9619
Uniprot ID	ABCG1_HUMAN
Specificity	This kit recognizes Human ABCG1 in samples. No significant cross-reactivity or interference between Human ABCG1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Tissue Specificity	Expressed in several tissues. Expressed in macrophages.expression is increased in macrophages from patients with Tangier disease.
Post Translational Modifications	Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.
Function	Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP. The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol.
Protein Name	Atp-Binding Cassette Sub-Family G Member 1 Atp-Binding Cassette Transporter 8 White Protein Homolog
Database Links	Reactome: R-HSA-1369062 Reactome: R-HSA-8964058 Reactome: R-HSA-9029569
Cellular Localisation	Endoplasmic Reticulum Membrane Multi-Pass Membrane Protein Golgi Apparatus Membrane Cell Membrane Predominantly Localized In The Intracellular Compartments Mainly Associated With The Endoplasmic Reticulum (Er) And Golgi Membranes
Alternative CLIA Names	Atp-Binding Cassette Sub-Family G Member 1 CLIA kit Atp-Binding Cassette Transporter 8 CLIA kit White Protein Homolog CLIA kit ABCG1 CLIA kit ABC8 CLIA kit WHT1 CLIA kit
Specificity	This kit recognizes Human ABCG1 in samples. No significant cross-reactivity or interference between Human ABCG1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-101	94
EDTA plasma(n=8)	87-101	93
Cell culture media(n=8)	85-98	90

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	98.36	258.81	673.22	101.87	238.81	618.16
Standard deviation	8.56	25.91	50.76	12.47	27.10	43.46
CV (%)	8.70	10.01	7.54	12.24	11.35	7.03

Citations

View product citations for antibody STJC0000299 on CiteAb