| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 11.37ng/mL |
| Detection Limit: | 31.2-2000ng/mL |
| Short Description : | This a1AT Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | SERPINA1 |
| Gene ID: | 5265 |
| Uniprot ID: | A1AT_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Ubiquitous. Expressed in leukocytes and plasma. |
| Post Translational Modifications | N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)Man(alpha1-3)Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases. |
| Function | Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). |
| Protein Name | Alpha-1-Antitrypsin Alpha-1 Protease Inhibitor Alpha-1-Antiproteinase Serpin A1 Cleaved Into - Short Peptide From Aat Spaat |
| Database Links | Reactome: R-HSA-114608 Reactome: R-HSA-204005 Reactome: R-HSA-381426 Reactome: R-HSA-5694530 Reactome: R-HSA-6798695 Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted Endoplasmic Reticulum The S And Z Allele Are Not Secreted Effectively And Accumulate Intracellularly In The Endoplasmic Reticulum Short Peptide From Aat: Secreted Extracellular Space Extracellular Matrix |
| Alternative ELISA Names | Alpha-1-Antitrypsin ELISA kit Alpha-1 Protease Inhibitor ELISA kit Alpha-1-Antiproteinase ELISA kit Serpin A1 Cleaved Into - Short Peptide From Aat ELISA kit Spaat ELISA kit SERPINA1 ELISA kit AAT ELISA kit PI ELISA kit PRO0684 ELISA kit PRO2209 ELISA kit |
| output |
Information sourced from Uniprot.org