| Host: | HEK293 cells NA |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human 2B4/CD244/SLAMF4 Protein-protein was developed from hek293 cells na na. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 µm filtered PBS solution pH7.4 |
| Dilution Range: | Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM |
| Storage Instruction: | The lyophilized protein is stable for at least 2 years from date of receipt at-20°C C. |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
| Gene Symbol: | CD244 |
| Gene ID: | 51744 |
| Uniprot ID: | CD244_HUMAN |
| Immunogen: | DNA sequence encoding Human 2B4/CD244/SLAMF4 including a C-hFc tag was expressed in HEK293 Cells. |
| Post Translational Modifications | N-linked glycosylation is essential for the binding to its ligand CD48. Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding. Phosphorylated by FYN and CSK on tyrosine residues following activation. Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells. |
| Function | Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family.its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor. Activating function implicates association with SH2D1A and FYN. Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2. Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis. Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells. Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1. At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells. Involved in the regulation of CD8(+) T-cell proliferation.expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells. Inhibits inflammatory responses in dendritic cells (DCs). |
| Protein Name | Natural Killer Cell Receptor 2b4 Nk Cell Activation-Inducing Ligand Nail Nk Cell Type I Receptor Protein 2b4 Nkr2b4 H2b4 Slam Family Member 4 Slamf4 Signaling Lymphocytic Activation Molecule 4 Cd Antigen Cd244 |
| Database Links | Reactome: R-HSA-202733 |
| Cellular Localisation | Membrane Single-Pass Type I Membrane Protein Cell Membrane Membrane Raft Receptor Engagement Results In A Recruitment To Lipid Drafts Essential For The Subsequent Tyrosine Phosphorylation Of The Itsms |
| Alternative Protein Names | Natural Killer Cell Receptor 2b4 protein Nk Cell Activation-Inducing Ligand protein Nail protein Nk Cell Type I Receptor Protein 2b4 protein Nkr2b4 protein H2b4 protein Slam Family Member 4 protein Slamf4 protein Signaling Lymphocytic Activation Molecule 4 protein Cd Antigen Cd244 protein CD244 protein 2B4 protein |
Information sourced from Uniprot.org