• Western blot analysis of extracts of HeLa cells, using GAPDH (STJ11103681) antibody as the primary antibody at dilution of 1:80000. Secondary antibody: using HRP Goat Anti-rabbit IgG (H+L) antibody (STJS000856) at 1:4000-1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Goat Anti-Rabbit IgG H&L antibody {HRP} (STJS000856)

SKU:
STJS000856

Current Stock:
Host: Goat
Applications: WB/DB/IHC-P/ELISA
Reactivity: Rabbit
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: HRP
Isotype: IgG
Formulation: PBS with 0.75% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:10000
DB:1:2000-1:10000
IHC-P:1:50-1:200
ELISA:1:5000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: Rabbit IgG
Background Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies. Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance