• Western blot analysis of extracts of HeLa cells, using GAPDH (STJ11103681) antibody as the primary antibody at dilution of 1:80000. Secondary antibody: using HRP Goat Anti-rabbit IgG (H+L) antibody (STJS000856) at 1:4000-1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Goat Anti-Rabbit IgG H&L antibody {HRP} (STJS000856)

SKU:
STJS000856-100

Current Stock:
Host: Goat
Applications: WB/IHC
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: HRP-conjugated goat anti-Rabbit IgG H&L secondary antibody. For use in most research applications.
Conjugation: HRP
Formulation: PBS with 0.75% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: ELISA 1:5000-1:10000
WB 1:2000-1:10000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Rabbit IgG
Immunogen Sequence: Email for sequence
Background This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.

Information sourced from Uniprot.org

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