| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Glucose Transporter GLUT4 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: | WB: 1:1, 000 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Slc2a4 |
| Gene ID: | 20528 |
| Uniprot ID: | GLUT4_MOUSE |
| Tissue Specificity | Expressed in skeletal and cardiac muscles. Expressed in brown and white adipose tissues. |
| Post Translational Modifications | Sumoylated. Palmitoylated. Palmitoylation by ZDHHC7 controls the insulin-dependent translocation of GLUT4 to the plasma membrane. |
| Function | Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell. |
| Peptide Name | Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4 Gt2 Glucose Transporter Type 4 - Insulin-Responsive Glut-4 |
| Database Links | Reactome: R-MMU-189200 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Endomembrane System Cytoplasm Perinuclear Region Localizes Primarily To The Perinuclear Region Undergoing Continued Recycling To The Plasma Membrane Where It Is Rapidly Reinternalized The Dileucine Internalization Motif Is Critical For Intracellular Sequestration Insulin Stimulation Induces Translocation To The Cell Membrane |
| Alternative Peptide Names | Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4 protein Gt2 protein Glucose Transporter Type 4 - Insulin-Responsive protein Glut-4 protein Slc2a4 protein Glut4 protein |
Information sourced from Uniprot.org