| Host: | E.coli |
| Applications: | SDS-PAGE |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-E.coli Dnak (508-638aa)-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications. |
| Formulation: | Liquid in 25mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 10% Glycerol |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: | 508-638aa |
| Accession Number: | NP_414555 |
| Immunogen: | E.coli |
| Immunogen Sequence: | MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIE SALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDV VDAEFEEVKD KK |
| Background | DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak (residues 508-638) of the substrate binding domain is alpha-helical and appears to act as a lid covering the substrate binding cleft. DnaK (amino acid 508-638) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N-terminus |
Information sourced from Uniprot.org