| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
DRP1 Positive Control for STJ500792 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:450 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Dnm1l |
| Gene ID: |
74006 |
| Uniprot ID: |
DNM1L_MOUSE |
| Specificity: |
This is positive control is recommended for use in combination with DRP1 antibody STJ500792. |
| Tissue Specificity | Expressed in the cerebellum and in several regions of the cerebrum and diencephalon. Strongly expressed in the cerebellar Purkinje cells and in the pontile giant neurons. |
| Post Translational Modifications | Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-643 inhibits mitochondrial fission probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-622 by Pink1 activates the GTPase activity and promotes mitochondrial fission. Phosphorylated in a circadian manner at Ser-643. Sumoylated on various lysine residues within the B domain, probably by MUL1. Sumoylation positively regulates mitochondrial fission. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity. S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage. O-GlcNAcylation augments the level of the GTP-bound active form of DNM1L and induces translocation from the cytoplasm to mitochondria in cardiomyocytes. It also decreases phosphorylation at Ser-643. Ubiquitination by MARCHF5 affects mitochondrial morphology. |
| Function | Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes. While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane. Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner. Plays an important role in mitochondrial fission during mitosis. Required for formation of endocytic vesicles. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis.this requirement may depend upon the cell type and the physiological apoptotic cues. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles.the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution. Rhythmic control of its activity following phosphorylation at Ser-643 is essential for the circadian control of mitochondrial ATP production. |
| Peptide Name | Dynamin-1-Like ProteinDynamin Family Member Proline-Rich Carboxyl-Terminal Domain LessDympleDynamin-Related Protein 1 |
| Database Links | Reactome: R-MMU-75153 |
| Cellular Localisation | CytoplasmCytosolGolgi ApparatusEndomembrane SystemPeripheral Membrane ProteinMitochondrion Outer MembranePeroxisomeMembraneClathrin-Coated PitCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneMainly CytosolicRecruited By Rala And Ralbp1 To Mitochondrion During MitosisTranslocated To The Mitochondrial Membrane Through O-Glcnacylation And Interaction With Fis1Colocalized With Marchf5 At Mitochondrial MembraneLocalizes To Mitochondria At Sites Of DivisionLocalizes To Mitochondria Following Necrosis InductionRecruited To The Mitochondrial Outer Membrane By Interaction With Mief1Mitochondrial Recruitment Is Inhibited By C11orf65/MfiAssociated With Peroxisomal MembranesPartly Recruited There By Pex11bMay Also Be Associated With Endoplasmic Reticulum Tubules And Cytoplasmic Vesicles And Found To Be PerinuclearIn Some Cell TypesLocalizes To The Golgi ComplexBinds To Phospholipid Membranes |
| Alternative Peptide Names | Dynamin-1-Like Protein proteinDynamin Family Member Proline-Rich Carboxyl-Terminal Domain Less proteinDymple proteinDynamin-Related Protein 1 proteinDnm1l proteinDrp1 protein |
Information sourced from Uniprot.org
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