| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Dnmt3a Positive Control for STJ506254 is synthetically produced from the 100-150 sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Dnmt3a |
| Gene ID: |
13435 |
| Uniprot ID: |
DNM3A_MOUSE |
| Immunogen Region: |
100-150 |
| Specificity: |
This is positive control is recommended for use in combination with Dnmt3a antibody STJ506254. |
| Post Translational Modifications | Auto-methylated at Cys-706: auto-methylation takes place in absence of DNA substrate and inactivates the DNA methyltransferase activity. Inactivation by auto-methylation may be used to inactivate unused DNA methyltransferases in the cell. Sumoylated.sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. |
| Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Also has weak auto-methylation activity on Cys-706 in absence of DNA. |
| Peptide Name | DnaCytosine-5-Methyltransferase 3aDnmt3aCysteine Methyltransferase Dnmt3aDna Methyltransferase MmuiiiaDna Mtase MmuiiiaM.mmuiiia |
| Database Links | Reactome: R-MMU-212300Reactome: -MMU-3214858 |
| Cellular Localisation | NucleusChromosomeCytoplasmAccumulates In The Major Satellite Repeats At Pericentric Heterochromatin |
| Alternative Peptide Names | Dna proteinCytosine-5-Methyltransferase 3a proteinDnmt3a proteinCysteine Methyltransferase Dnmt3a proteinDna Methyltransferase Mmuiiia proteinDna Mtase Mmuiiia proteinM.mmuiiia proteinDnmt3a protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
