Deformed Epidermal Autoregulatory Factor 1 Blocking Peptide for STJ500748 is synthetically produced from the 500-565 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide taken within amino acid region 500-565 on mouse Deformed epidermal autoregulatory factor 1 homolog protein.
Immunogen Region
500-565
Specificity
This blocking peptide is recommended for use in combination with Deformed Epidermal Autoregulatory Factor 1 antibody, STJ500748
Additional Info
Tissue Specificity
Ubiquitously expressed during embryogenesis, with higher expression in regions of the central nervous system, dorsal root ganglia, submandibular gland, epidermis and breast. In 12-week-old NOD mice, expression of isoform 2 is sevenfold higher in lymph node stromal elements than in T-cells and tenfold higher than in B-cells.
Post Translational Modifications
May be phosphorylated by DNA-PK complex in a DNA independent manner (in vitro).
Function
Transcription factor that binds to sequence with multiple copies of 5'-TTCCGG-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. Regulates epithelial cell proliferation and side-branching in the mammary gland. Required for neural tube closure and skeletal patterning. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 2. Isoform 2 may inhibit transcriptional activity of isoform 1 by interacting with it and retaining it in the cytoplasm. Transcriptional activator of EIF4G3. May also involved in behavior.
Isoform 1: NucleusCytoplasmIsoform 2: CytoplasmNucleusDisplays Some Nuclear Localization When Expressed With Isoform 1Suggesting That It May Heterodimerize With Isoform 1 And Shuttle To The Nucleus Using The Nuclear Localization Signal Of Isoform 1
