canine IL-8/CXCL8 protein (Recombinant) (His-Tag) (STJP019480)
SPECIFICATIONS
HostHEK293 Cells
ImmunogenCanine
General Information
| Short Description | Recombinant-canine IL-8/CXCL8-His-Tag protein was developed from hek293 cells and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | HEK293 Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid In Phosphate-Buffered Saline (pH 7.4) containing 20% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
Target Information
| Accession Number | NP_001003200.1 |
| Immunogen | Canine |
| Immunogen Region | 28-101aa |
| Immunogen Sequence | VSSELRCQCI KTHSTPFHPK YIKELRVIDS GPHCENSEII VKLFNGNEVC LDPKEKWVQK VVQIFLKKAE KQDP |
Additional Info
| Background | IL-8/CXCL8, also known as Interleukin-8, GCP-1, and NAP-1 is a member of the CXC family of chemokines family which is widely expressed proinflammatory. IL-8 is a potent neutrophil chemotactic factor. It attracts neutrophils, basophils, and T-cells, but not monocytes. This protein has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also stimulates phagocytosis once they have arrived. IL-8 is also known to be a potent promoter of angiogenesis. In target cells, IL-8 induces a series of physiological responses required for migration and phagocytosis, such as increases in intracellular Ca2+, exocytosis, and the respiratory burst. Among the eceptors on the surface membrane capable of binding IL-8, the most frequently studied receptors are CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). Recombinant canine IL-8/CXCL8, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques. |
Information sourced from Uniprot.org