BCKDH Blocking Peptide peptide (STJ503694)

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STJ503694-250
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Applications: Immunodepletion/Immunocompetition
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : BCKDH Blocking Peptide is synthetically produced from the 170-200 sequence and is suitable for use in western blot applications.
Formulation: Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: BCKDK
Gene ID: 10295
Uniprot ID: BCKD_HUMAN
Immunogen Region: 170-200
Immunogen: Synthetic peptide taken within amino acid region 170-200 on human BCKD protein.
Tissue Specificity Ubiquitous.
Post Translational Modifications Autophosphorylated.
Function Serine/threonine-protein kinase component of macronutrients metabolism. Forms a functional kinase and phosphatase pair with PPM1K, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle. Phosphorylates and inactivates mitochondrial BCKDH complex a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism: E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Associates with the E2 component of BCKDH complex and phosphorylates BCKDHA on Ser-337, leading to conformational changes that interrupt substrate channeling between E1 and E2 and inactivates the BCKDH complex. Phosphorylates ACLY on Ser-455 in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and glucogenesis, respectively. Recognizes phosphosites having SxxE/D canonical motif.
Peptide Name Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase
Bckdh Kinase
Bckdhkin
Bdk
3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial
Database Links Reactome: R-HSA-70895
Cellular Localisation Mitochondrion Matrix
Detected In The Cytosolic Compartment Of Liver Cells
Alternative Peptide Names Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase protein
Bckdh Kinase protein
Bckdhkin protein
Bdk protein
3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial protein
BCKDK protein

Information sourced from Uniprot.org

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