| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
ARF1 Blocking Peptide for STJ500153 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Arf1 |
| Gene ID: |
64310 |
| Uniprot ID: |
ARF1_RAT |
| Specificity: |
This blocking peptide is recommended for use in combination with ARF1 antibody, STJ500153 |
| Immunogen: |
Synthetic peptide corresponding to unique amino acid sequence on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desied antigenicity. |
| Function | Small GTPase involved in protein trafficking between different compartments. Modulates vesicle budding and uncoating within the Golgi complex. In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity.the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD). |
| Peptide Name | Adp-Ribosylation Factor 1 |
| Database Links | Reactome: R-RNO-1660499Reactome: -RNO-1660514Reactome: -RNO-199992Reactome: -RNO-2132295Reactome: -RNO-432720Reactome: -RNO-432722Reactome: -RNO-6807878Reactome: -RNO-6811434Reactome: -RNO-6811438 |
| Cellular Localisation | Golgi Apparatus MembraneLipid-AnchorCytoplasmic SideSynapseSynaptosomePostsynaptic DensityIn The Gdp-Bound FormAssociates Transiently With The Membranes Via Its Myristoylated N-Terminus Where Guanine Nucleotide-Exchange Factor (Gef)-Mediated Nucleotide Exchange OccursFollowing Nucleotide ExchangeThe Gtp-Bound Form Undergoes A Conformational ChangeLeading To The Exposure Of A Myristoylated N-Terminal Amphipathic Helix That Provides Stable Membrane Anchorage |
| Alternative Peptide Names | Adp-Ribosylation Factor 1 proteinArf1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
