| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ZPBP |
| Gene ID: | 11055 |
| Uniprot ID: | ZPBP1_HUMAN |
| Immunogen Region: | 45-200 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 45-200 of human ZPBP (NP_008940.2). |
| Immunogen Sequence: | HLVRLPRAFRLTKDSVKIVG STSFPVKAYVMLHQKSPHVL CVTQQLRNAELIDPSFQWYG PKGKVVSVENRTAQITSTGS LVFQNFEESMSGIYTCFLEY KPTVEEIVKRLQLKYAIYAY REPHYYYQFTARYHAAPCNS IYNISFEKKLLQILSK |
| Tissue Specificity | Expressed specifically in testis. |
| Post Translational Modifications | N-glycosylated. |
| Function | Plays a role in acrosome compaction and sperm morphogenesis. Is implicated in sperm-oocyte interaction during fertilization. |
| Protein Name | Zona Pellucida-Binding Protein 1Inner Acrosomal Membrane Iam38Sp38 |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleAcrosomeAcrosome MembranePeripheral Membrane ProteinSecretedFirst Localized In Acrosome GranuleLater Migrates To The Inner And Outer Acrosomal MembraneReleased After The Acrosomal Reaction |
| Alternative Antibody Names | Anti-Zona Pellucida-Binding Protein 1 antibodyAnti-Inner Acrosomal Membrane Iam38 antibodyAnti-Sp38 antibodyAnti-ZPBP antibodyAnti-ZPBP1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and CD147/CD147/BSG knockout (KO) 293T cells, using [KO Validated] CD147/BSG Rabbit polyclonal antibody (STJ11100005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89078/357535/STJ11100005_1__56198.1713121650.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CD147/CD147/BSG knockout (KO) 293T cells, using [KO Validated] CD147/BSG Rabbit polyclonal antibody (STJ11100005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
