Western blot analysis of various lysates using ZP1 Rabbit polyclonal antibody (STJ112672) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ZP1 antibody (340-610) (STJ112672)

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STJ112672
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZP1
Gene ID: 22917
Uniprot ID: ZP1_HUMAN
Immunogen Region: 340-610
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 340-610 of human ZP1 (NP_997224.2).
Immunogen Sequence: AGDQLIYENWLVSGIHIQKG PQGSITRDSTFQLHVRCVFN ASDFLPIQASIFPPPSPAPM TQPGPLRLELRIAKDETFSS YYGEDDYPIVRLLREPVHVE VRLLQRTDPNLVLLLHQCWG APSANPFQQPQWPILSDGCP FKGDSYRTQMVALDGATPFQ SHYQRFTVATFALLDSGSQR ALRGLVYLFCSTSACHTSGL ETCSTACSTGTTRQRRSSGH RNDTARPQDIVSSPGPVGF
Tissue Specificity Expressed in oocytes (at protein level).
Post Translational Modifications Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. O-glycosylated.
Function Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.
Protein Name Zona Pellucida Sperm-Binding Protein 1
Zona Pellucida Glycoprotein 1
Zp-1 Cleaved Into - Processed Zona Pellucida Sperm-Binding Protein 1
Database Links Reactome: R-HSA-2534343
Cellular Localisation Processed Zona Pellucida Sperm-Binding Protein 1: Zona Pellucida
Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Zona Pellucida Sperm-Binding Protein 1 antibody
Anti-Zona Pellucida Glycoprotein 1 antibody
Anti-Zp-1 Cleaved Into - Processed Zona Pellucida Sperm-Binding Protein 1 antibody
Anti-ZP1 antibody

Information sourced from Uniprot.org

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