Anti-ZO1 antibody (STJ13100477)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from mouse ZO1 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-ZO1 for use in IHC and WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Tjp1 |
| Gene ID | 21872 |
| Uniprot ID | ZO1_MOUSE |
| Immunogen | A synthetic peptide from mouse ZO1 conjugated to blue carrier protein was used as the antigen. |
| Specificity | Specific for ZO1. |
Additional Info
| Post Translational Modifications | Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ. |
| Function | Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With Tjp2 and TJjp3, participates in the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus. May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions. |
| Protein Name | Tight Junction Protein 1Tight Junction Protein Zo-1Zona Occludens Protein 1Zonula Occludens Protein 1 |
| Database Links | Reactome: R-MMU-191650Reactome: -MMU-2028269Reactome: -MMU-351906 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionTight JunctionGap JunctionCytoplasmMyofibrilSarcomereI BandMoves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell ContactForms A Condensed Tight Junction-Linked Belt Of Protein During Junction Formation Which Becomes Anchored To The Apical Cell Membrane Via Interaction With PatjDetected At The Leading Edge Of Migrating And Wounded CellsColocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells |
| Alternative Antibody Names | Anti-Tight Junction Protein 1 antibodyAnti-Tight Junction Protein Zo-1 antibodyAnti-Zona Occludens Protein 1 antibodyAnti-Zonula Occludens Protein 1 antibodyAnti-Tjp1 antibodyAnti-Zo1 antibody |
Information sourced from Uniprot.org