Host: | Goat |
Applications: | Pep-ELISA/WB/IHC |
Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Goat polyclonal antibody anti-ZNRF1-Mouse (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 28kDa band observed in Mouse embryo and adult Brain lysates (right panel) , consistent with the observed band in transfected N2a cells transiently expressing Znrf1 (left panel) |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | ZNRF1 |
Gene ID: | 84937 |
Uniprot ID: | ZNRF1_HUMAN |
Immunogen Region: | Internal |
Accession Number: | NP_573469.1; NP_001162093.1 |
Specificity: | This antibody is expected to recognize reported isoforms a and b (NP_573469.1; NP_001162093.1). Reported variants represent identical protein: NP_001162092.1, NP_573469.1 |
Immunogen Sequence: | SDSTYAHGNGYQET |
Post Translational Modifications | N-myristoylation targets ZNRF1 to intracellular membranes. Phosphorylated by SRC at Tyr-103.leading to 'Lys-63'-linked ubiquitination of TLR3, lysosomal trafficking and degradation. |
Function | E3 ubiquitin-protein ligase that plays a role in different processes including cell differentiation, receptor recycling or regulation of inflammation. Mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons. Ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit/ATP1A1 and thereby influences its endocytosis and/or degradation. Controls ligand-induced EGFR signaling via mediating receptor ubiquitination and recruitment of the ESCRT machinery. Acts as a negative feedback mechanism controlling TLR3 trafficking by mediating TLR3 'Lys-63'-linked polyubiquitination to reduce type I IFN production. Modulates inflammation by promoting caveolin-1/CAV1 ubiquitination and degradation to regulate TLR4-activated immune response. |
Protein Name | E3 Ubiquitin-Protein Ligase Znrf1 Nerve Injury-Induced Gene 283 Protein Ring-Type E3 Ubiquitin Transferase Znrf1 Zinc/Ring Finger Protein 1 |
Database Links | Reactome: R-HSA-983168 |
Cellular Localisation | Endosome Lysosome Membrane Peripheral Membrane Protein Cytoplasmic Vesicle Secretory Vesicle Synaptic Vesicle Membrane Associated With Synaptic Vesicle Membranes In Neurons |
Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Znrf1 antibody Anti-Nerve Injury-Induced Gene 283 Protein antibody Anti-Ring-Type E3 Ubiquitin Transferase Znrf1 antibody Anti-Zinc/Ring Finger Protein 1 antibody Anti-ZNRF1 antibody Anti-NIN283 antibody |
Information sourced from Uniprot.org