• N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with STJ72828 (1µg/ml) , also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ZNRF1-Mouse antibody (Internal) (STJ72828)

SKU:
STJ72828-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ZNRF1-Mouse (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 28kDa band observed in Mouse embryo and adult Brain lysates (right panel) , consistent with the observed band in transfected N2a cells transiently expressing Znrf1 (left panel)
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ZNRF1
Gene ID: 84937
Uniprot ID: ZNRF1_HUMAN
Immunogen Region: Internal
Accession Number: NP_573469.1; NP_001162093.1
Specificity: This antibody is expected to recognize reported isoforms a and b (NP_573469.1; NP_001162093.1). Reported variants represent identical protein: NP_001162092.1, NP_573469.1
Immunogen Sequence: SDSTYAHGNGYQET
Post Translational Modifications N-myristoylation targets ZNRF1 to intracellular membranes. Phosphorylated by SRC at Tyr-103.leading to 'Lys-63'-linked ubiquitination of TLR3, lysosomal trafficking and degradation.
Function E3 ubiquitin-protein ligase that plays a role in different processes including cell differentiation, receptor recycling or regulation of inflammation. Mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons. Ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit/ATP1A1 and thereby influences its endocytosis and/or degradation. Controls ligand-induced EGFR signaling via mediating receptor ubiquitination and recruitment of the ESCRT machinery. Acts as a negative feedback mechanism controlling TLR3 trafficking by mediating TLR3 'Lys-63'-linked polyubiquitination to reduce type I IFN production. Modulates inflammation by promoting caveolin-1/CAV1 ubiquitination and degradation to regulate TLR4-activated immune response.
Protein Name E3 Ubiquitin-Protein Ligase Znrf1
Nerve Injury-Induced Gene 283 Protein
Ring-Type E3 Ubiquitin Transferase Znrf1
Zinc/Ring Finger Protein 1
Database Links Reactome: R-HSA-983168
Cellular Localisation Endosome
Lysosome
Membrane
Peripheral Membrane Protein
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Associated With Synaptic Vesicle Membranes In Neurons
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Znrf1 antibody
Anti-Nerve Injury-Induced Gene 283 Protein antibody
Anti-Ring-Type E3 Ubiquitin Transferase Znrf1 antibody
Anti-Zinc/Ring Finger Protein 1 antibody
Anti-ZNRF1 antibody
Anti-NIN283 antibody

Information sourced from Uniprot.org