Anti-ZNF364 antibody [R06-1B3] (STJA0033782)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human ZNF364
General Information
| Short Description | Rabbit monoclonal anti-ZNF364 for use in WB in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R06-1B3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | RNF115 |
| Gene ID | 27246 |
| Uniprot ID | RN115_HUMAN |
| Immunogen | A synthetic peptide of human ZNF364 |
Additional Info
| Tissue Specificity | Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells. |
| Post Translational Modifications | Phosphorylated by AKT1, allowing association with the 14-3-3 chaperones that facilitates associating with TLRs. RING-type zinc finger-dependent and E2-dependent autoubiquitination. Deubiquitinated by USP9X.antogonizing its autoubiquitination and subsequent proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase that catalyzes the 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of various substrates and thereby plays a role in a number of signaling pathways including autophagy, innate immunity, cell proliferation and cell death. Plays a role in the endosomal trafficking and degradation of membrane receptors including EGFR, FLT3, MET and CXCR4 through their polyubiquitination. Participates together with BST2 in antiviral immunity by facilitating the internalization of HIV-1 virions into intracellular vesicles leading to their lysosomal degradation. Also possesses an antiviral activity independently of BST2 by promoting retroviral GAG proteins ubiquitination, redistribution to endo-lysosomal compartments and, ultimately, lysosomal degradation. Catalyzes distinct types of ubiquitination on MAVS and STING1 at different phases of viral infection to promote innate antiviral response. Mediates the 'Lys-48'-linked ubiquitination of MAVS leading to its proteasomal degradation and ubiquitinates STING1 via 'Lys-63'-linked polyubiquitination, critical for its oligomerization and the subsequent recruitment of TBK1. Plays a positive role in the autophagosome-lysosome fusion by interacting with STX17 and enhancing its stability without affecting 'Lys-48'- or 'Lys-63'-linked polyubiquitination levels, which in turn promotes autophagosome maturation. Negatively regulates TLR-induced expression of proinflammatory cytokines by catalyzing 'Lys-11'-linked ubiquitination of RAB1A and RAB13 to inhibit post-ER trafficking of TLRs to the Golgi by RAB1A and subsequently from the Golgi apparatus to the cell surface by RAB13. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf115Ring Finger Protein 115Ring-Type E3 Ubiquitin Transferase Rnf115Rab7-Interacting Ring Finger ProteinRabring 7Zinc Finger Protein 364 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusEndoplasmic ReticulumGolgi ApparatusThe Gtp-Bound Form Of Rab7a Recruits Rnf115 From The Cytosol Onto Late Endosomes/Lysosomes |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf115 antibodyAnti-Ring Finger Protein 115 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf115 antibodyAnti-Rab7-Interacting Ring Finger Protein antibodyAnti-Rabring 7 antibodyAnti-Zinc Finger Protein 364 antibodyAnti-RNF115 antibodyAnti-ZNF364 antibody |
Information sourced from Uniprot.org