• Immunohistochemistry analysis of paraffin-embedded Human colon using ZMPSTE24 Rabbit polyclonal antibody (STJ11100539) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat ovary using ZMPSTE24 Rabbit polyclonal antibody (STJ11100539) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Mouse heart using ZMPSTE24 Rabbit polyclonal antibody (STJ11100539) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of SKOV3 cells, using ZMPSTE24 antibody (STJ11100539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ZMPSTE24 antibody (200-300) (STJ11100539)

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STJ11100539

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZMPSTE24 (200-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZMPSTE24
Gene ID: 10269
Uniprot ID: FACE1_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ZMPSTE24 (NP_005848.2).
Immunogen Sequence: AWLFTLVVSLVLVTIYADYI APLFDKFTPLPEGKLKEEIE VMAKSIDFPLTKVYVVEGSK RSSHSNAYFYGFFKNKRIVL FDTLLEEYSVLNKDIQEDSG M
Tissue Specificity Widely expressed. High levels in kidney, prostate, testis and ovary.
Function Transmembrane metalloprotease whose catalytic activity is critical for processing lamin A/LMNA on the inner nuclear membrane and clearing clogged translocons on the endoplasmic reticulum. Proteolytically removes the C-terminal three residues of farnesylated proteins. Plays also an antiviral role independently of its protease activity by restricting enveloped RNA and DNA viruses, including influenza A, Zika, Ebola, Sindbis, vesicular stomatitis, cowpox, and vaccinia. Mechanistically, controls IFITM antiviral pathway to hinder viruses from breaching the endosomal barrier by modulating membrane fluidity.
Protein Name Caax Prenyl Protease 1 Homolog
Farnesylated Proteins-Converting Enzyme 1
Face-1
Prenyl Protein-Specific Endoprotease 1
Zinc Metalloproteinase Ste24 Homolog
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Nucleus Inner Membrane
Early Endosome Membrane
Late Endosome Membrane
Alternative Antibody Names Anti-Caax Prenyl Protease 1 Homolog antibody
Anti-Farnesylated Proteins-Converting Enzyme 1 antibody
Anti-Face-1 antibody
Anti-Prenyl Protein-Specific Endoprotease 1 antibody
Anti-Zinc Metalloproteinase Ste24 Homolog antibody
Anti-ZMPSTE24 antibody
Anti-FACE1 antibody
Anti-STE24 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance