• Western blot analysis of extracts of various cell lines, using ZIC3 antibody (STJ111714) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using ZIC3 antibody (STJ111714) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ZIC3 antibody (1-240) (STJ111714)

SKU:
STJ111714

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZIC3 (1-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZIC3
Gene ID: 7547
Uniprot ID: ZIC3_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZIC3 (NP_003404.1).
Immunogen Sequence: MTMLLDGGPQFPGLGVGSFG APRHHEMPNREPAGMGLNPF GDSTHAAAAAAAAAAFKLSP AAAHDLSSGQSSAFTPQGSG YANALGHHHHHHHHHHHTSQ VPSYGGAASAAFNSTREFLF RQRSSGLSEAASGGGQHGLF AGSASSLHAPAGIPEPPSYL LFPGLHEQGAGHPSPTGHVD NNQVHLGLRGELFGRADPYR PVASPRTDPYAAGAQFPNYS PMNMNMGVNVAAHHGPGAF
Function Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Protein Name Zinc Finger Protein Zic 3
Zinc Finger Protein 203
Zinc Finger Protein Of The Cerebellum 3
Database Links Reactome: R-HSA-2892247
Reactome: R-HSA-452723
Cellular Localisation Nucleus
Cytoplasm
Localizes In The Cytoplasm In Presence Of Mdfic Overexpression
Translocation To The Nucleus Requires Kpna1 Or Kpna6
Alternative Antibody Names Anti-Zinc Finger Protein Zic 3 antibody
Anti-Zinc Finger Protein 203 antibody
Anti-Zinc Finger Protein Of The Cerebellum 3 antibody
Anti-ZIC3 antibody
Anti-ZNF203 antibody

Information sourced from Uniprot.org

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