| Host: |
Rabbit |
| Applications: |
IHC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Mrna Decay Activator Protein Zfp36 is suitable for use in Immunohistochemistry and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4 |
| Purification: |
Antigen affinity purification |
| Concentration: |
0.8 mg/mL |
| Dilution Range: |
IHC 1:40-1:200 |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Gene Symbol: |
ZFP36 |
| Gene ID: |
7538 |
| Uniprot ID: |
TTP_HUMAN |
| Immunogen: |
Fusion protein of human ZFP36 |
| Tissue Specificity | Expressed in both basal and suprabasal epidermal layers. Expressed in epidermal keratinocytes. Expressed strongly in mature dendritic cells. Expressed in immature dendritic cells (at protein level). |
| Post Translational Modifications | Phosphorylated. Phosphorylation at serine and/or threonine residues occurs in a p38 MAPK- and MAPKAPK2-dependent manner. Phosphorylated by MAPKAPK2 at Ser-60 and Ser-186.phosphorylation increases its stability and cytoplasmic localization, promotes binding to 14-3-3 adapter proteins and inhibits the recruitment of cytoplasmic CCR4-NOT and PAN2-PAN3 deadenylase complexes to the mRNA decay machinery, thereby inhibiting ZFP36-induced ARE-containing mRNA deadenylation and decay processes. Phosphorylation by MAPKAPK2 does not impair ARE-containing RNA-binding. Phosphorylated in a MAPKAPK2- and p38 MAPK-dependent manner upon skeletal muscle satellite cell activation.this phosphorylation inhibits ZFP36-mediated mRNA decay activity, and hence stabilizes MYOD1 mRNA. Phosphorylated by MAPK1 upon mitogen stimulation. Phosphorylated at Ser-66 and Ser-93.these phosphorylations increase in a SH3KBP1-dependent manner. Phosphorylated at serine and threonine residues in a pyruvate kinase PKM- and p38 MAPK-dependent manner. Phosphorylation at Ser-60 may participate in the PKM-mediated degradation of ZFP36 in a p38 MAPK-dependent manner. Dephosphorylated by serine/threonine phosphatase 2A at Ser-186. Ubiquitinated.pyruvate kinase (PKM)-dependent ubiquitination leads to proteasomal degradation through a p38 MAPK signaling pathway. |
| Function | Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as an 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1, and hence promotes ARE-mediated mRNA deadenylation. Functions also by recruiting components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs. Self regulates by destabilizing its own mRNA. Binds to 3'-UTR ARE of numerous mRNAs and of its own mRNA. Plays a role in anti-inflammatory responses.suppresses tumor necrosis factor (TNF)-alpha production by stimulating ARE-mediated TNF-alpha mRNA decay and several other inflammatory ARE-containing mRNAs in interferon (IFN)- and/or lipopolysaccharide (LPS)-induced macrophages. Also plays a role in the regulation of dendritic cell maturation at the post-transcriptional level, and hence operates as part of a negative feedback loop to limit the inflammatory response. Promotes ARE-mediated mRNA decay of hypoxia-inducible factor HIF1A mRNA during the response of endothelial cells to hypoxia. Positively regulates early adipogenesis of preadipocytes by promoting ARE-mediated mRNA decay of immediate early genes (IEGs). Negatively regulates hematopoietic/erythroid cell differentiation by promoting ARE-mediated mRNA decay of the transcription factor STAT5B mRNA. Plays a role in maintaining skeletal muscle satellite cell quiescence by promoting ARE-mediated mRNA decay of the myogenic determination factor MYOD1 mRNA. Associates also with and regulates the expression of non-ARE-containing target mRNAs at the post-transcriptional level, such as MHC class I mRNAs. Participates in association with argonaute RISC catalytic components in the ARE-mediated mRNA decay mechanism.assists microRNA (miRNA) targeting ARE-containing mRNAs. May also play a role in the regulation of cytoplasmic mRNA decapping.enhances decapping of ARE-containing RNAs, in vitro. Involved in the delivery of target ARE-mRNAs to processing bodies (PBs). In addition to its cytosolic mRNA-decay function, affects nuclear pre-mRNA processing. Negatively regulates nuclear poly(A)-binding protein PABPN1-stimulated polyadenylation activity on ARE-containing pre-mRNA during LPS-stimulated macrophages. Also involved in the regulation of stress granule (SG) and P-body (PB) formation and fusion. Plays a role in the regulation of keratinocyte proliferation, differentiation and apoptosis. Plays a role as a tumor suppressor by inhibiting cell proliferation in breast cancer cells. (Microbial infection) Negatively regulates HTLV-1 TAX-dependent transactivation of viral long terminal repeat (LTR) promoter. |
| Protein Name | Mrna Decay Activator Protein Zfp36G0/G1 Switch Regulatory Protein 24Growth Factor-Inducible Nuclear Protein Nup475TristetraprolinZinc Finger Protein 36Zfp-36 |
| Database Links | Reactome: R-HSA-450513 |
| Cellular Localisation | NucleusCytoplasmCytoplasmic GranuleP-BodyShuttles Between Nucleus And Cytoplasm In A Crm1-Dependent MannerLocalized Predominantly In The Cytoplasm In A P38 Mapk- And Ywhab-Dependent MannerColocalizes With Sh3kbp1 And Map3k4 In The CytoplasmComponent Of Cytoplasmic Stress Granules (Sgs)Localizes To Cytoplasmic Stress Granules Upon Energy StarvationLocalizes In Processing Bodies (Pbs)Excluded From Stress Granules In A Phosphorylation Mapkapk2-Dependent MannerShuttles In And Out Of Both Cytoplasmic P-Body And Sgs(Microbial Infection) Colocalizes With Htlv-1 Tax In The Nucleus And The Cytoplasm In A Region Surrounding The Nucleus |
| Alternative Antibody Names | Anti-Mrna Decay Activator Protein Zfp36 antibodyAnti-G0/G1 Switch Regulatory Protein 24 antibodyAnti-Growth Factor-Inducible Nuclear Protein Nup475 antibodyAnti-Tristetraprolin antibodyAnti-Zinc Finger Protein 36 antibodyAnti-Zfp-36 antibodyAnti-ZFP36 antibodyAnti-G0S24 antibodyAnti-NUP475 antibodyAnti-RNF162A antibodyAnti-TIS11A antibodyAnti-TTP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
