| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S5649RM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:20000-1:40000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ZEB1 |
| Gene ID: | 6935 |
| Uniprot ID: | ZEB1_HUMAN |
| Immunogen Region: | 800-900 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 800-900 of human ZEB1 (NP_110378.3). |
| Immunogen Sequence: | INIAIPTVTAQLPTIVAIAD QNSVPCLRALAANKQTILIP QVAYTYSTTVSPAVQEPPLK VIQPNGNQDERQDTSSEGVS NVEDQNDSDSTPPKKKMRKT E |
| Tissue Specificity | Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. |
| Post Translational Modifications | Ubiquitinated, leading to degradation in a proteasome-dependent manner. Deubiquitinated by USP51, leading to stabilization. |
| Function | Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region. |
| Protein Name | Zinc Finger E-Box-Binding Homeobox 1 Nil-2-A Zinc Finger Protein Negative Regulator Of Il2 Transcription Factor 8 Tcf-8 |
| Database Links | Reactome: R-HSA-6785807 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Zinc Finger E-Box-Binding Homeobox 1 antibody Anti-Nil-2-A Zinc Finger Protein antibody Anti-Negative Regulator Of Il2 antibody Anti-Transcription Factor 8 antibody Anti-Tcf-8 antibody Anti-ZEB1 antibody Anti-AREB6 antibody Anti-TCF8 antibody |
Information sourced from Uniprot.org