| Short Description : | Rabbit polyclonal ZBTB33 (523-672) antibody for use in WB, ELISA and ChIP in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA/ChIP |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.ChIP:5 Mu g antibody for 10 Mu g-15 Mu g of Chromatin |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ZBTB33 |
| Gene ID: | 10009 |
| Uniprot ID: | KAISO_HUMAN |
| Immunogen Region: | 523-672 |
| Immunogen Sequence: | PCRYCEKVFPLAEYRTKHEI HHTGERRYQCLACGKSFINY QFMSSHIKSVHSQDPSGDSK LYRLHPCRSLQIRQYAYLSD RSSTIPAMKDDGIGYKVDTG KEPPVGTTTSTQNKPMTWED IFIQQENDSIFKQNVTDGST EFEFIIPESY |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 523-672 of human Kaiso/Kaiso/ZBTB33 (NP_006768.1). |
| Tissue Specificity | Expressed in vascular endothelium. |
| Function | Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1. |
| Protein Name | Transcriptional Regulator KaisoZinc Finger And Btb Domain-Containing Protein 33 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmAlso Cytoplasmic In Cells Grown At High Densities |
| Alternative Antibody Names | Anti-Transcriptional Regulator Kaiso antibodyAnti-Zinc Finger And Btb Domain-Containing Protein 33 antibodyAnti-ZBTB33 antibodyAnti-KAISO antibodyAnti-ZNF348 antibody |
Information sourced from Uniprot.org
