Anti-ZBED1 antibody (1-280) (STJ28875)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ZBED1 (1-280) antibody for use in WB, IHC-P, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ZBED1 |
| Gene ID | 9189 |
| Uniprot ID | ZBED1_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MENKSLESSQTDLKLVAHPR AKSKVWKYFGFDTNAEGCIL QWKKIYCRICMAQIAYSGNT SNLSYHLEKNHPEEFCEFVK SNTEQMREAFATAFSKLKPE SSQQPGQDALAVKAGHGYDS KKQQELTAAVLGLICEGLYP ASIVDEPTFKVLLKTADPRY ELPSRKYISTKAIPEKYGAV REVILKELAEATWCGISTDM WRSENQNRAYVTLAAHFLGL GAPNCLSMGSRCLKTFEVP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZBED1 (NP_001164607.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed at low levels. Expression is highest in skeletal muscle, heart, spleen and placenta. |
| Post Translational Modifications | Autosumoylated with SUMO1, SUMO2, and SUMO3. |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA. This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as: RPS6, RPL10A, and RPL12. The resulting increased transcriptional activity drives cell proliferation. Binds to 5'-TGTCGCTGACTA-3' consensus sequences in gene promoters of ribosomal proteins. (Microbial infection) Binds to human adenovirus gene promoters and contributes to transcriptional repression and virus growth inhibition during early stages of infection. |
| Protein Name | E3 Sumo-Protein Ligase Zbed1Dna Replication-Related Element-Binding FactorPutative Ac-Like Transposable ElementZinc Finger Bed Domain-Containing Protein 1Dref Homolog |
| Database Links | Reactome: R-HSA-4551638 |
| Cellular Localisation | NucleusPml BodyIn Granular Structures(Microbial Infection) Upon Interaction With Human Adenovirus Early E1a ProteinThe Protein Is Redistributed To The Peripheral Areas Of Pml Bodies |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Zbed1 antibodyAnti-Dna Replication-Related Element-Binding Factor antibodyAnti-Putative Ac-Like Transposable Element antibodyAnti-Zinc Finger Bed Domain-Containing Protein 1 antibodyAnti-Dref Homolog antibodyAnti-ZBED1 antibodyAnti-ALTE antibodyAnti-DREF antibodyAnti-hDREF antibodyAnti-KIAA0785 antibodyAnti-TRAMP antibody |
Information sourced from Uniprot.org