| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-YWHAZ (146-245) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
YWHAZ |
| Gene ID: |
7534 |
| Uniprot ID: |
1433Z_HUMAN |
| Immunogen Region: |
146-245 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 146-245 of human YWHAZ (NP_003397.1). |
| Immunogen Sequence: |
QQAYQEAFEISKKEMQPTHP IRLGLALNFSVFYYEILNSP EKACSLAKTAFDEAIAELDT LSEESYKDSTLIMQLLRDNL TLWTSDTQGDEAEAGEGGEN |
| Post Translational Modifications | The delta, brain-specific form differs from the zeta form in being phosphorylated (Probable). Phosphorylation on Ser-184 by MAPK8.promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232.inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA.disrupts homodimerization and heterodimerization with YHAE and TP53. |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, regulates spine maturation through the modulation of ARHGEF7 activity. |
| Protein Name | 14-3-3 Protein Zeta/DeltaProtein Kinase C Inhibitor Protein 1Kcip-1 |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-1445148Reactome: R-HSA-3769402Reactome: R-HSA-392517Reactome: R-HSA-430116Reactome: R-HSA-450604Reactome: R-HSA-512988Reactome: R-HSA-5625740Reactome: R-HSA-5628897Reactome: R-HSA-75035Reactome: R-HSA-9013700Reactome: R-HSA-9604323Reactome: R-HSA-9614399Reactome: R-HSA-9735871Reactome: R-HSA-9755779 |
| Cellular Localisation | CytoplasmMelanosomeLocated To Stage I To Stage Iv Melanosomes |
| Alternative Antibody Names | Anti-14-3-3 Protein Zeta/Delta antibodyAnti-Protein Kinase C Inhibitor Protein 1 antibodyAnti-Kcip-1 antibodyAnti-YWHAZ antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
