| Short Description : | Rabbit polyclonal YTHDC2 (1131-1430) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | YTHDC2 |
| Gene ID: | 64848 |
| Uniprot ID: | YTDC2_HUMAN |
| Immunogen Region: | 1131-1430 |
| Immunogen Sequence: | HSLFLRRMRAPSKPWSQVDE ATIRAIIAVLSTEEQSAGLQ QPSGIGQRPRPMSSEELPLA SSWRSNNSRKSSADTEFSDE CTTAERVLMKSPSPALHPPQ KYKDRGILHPKRGTEDRSDQ SSLKSTDSSSYPSPCASPSP PSSGKGSKSPSPRPNMPVRY FIMKSSNLRNLEISQQKGIW STTPSNERKLNRAFWESSIV YLVFSVQGSGHFQGFSRMSS EIGREKSQDWGSAGLGGVF |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1131-1430 of human YTHDC2 (NP_073739.3). |
| Tissue Specificity | Expressed in testis. Not detected in spermatogonia next to the tubule wall but is strongly expressed in spermatocytes, suggesting that it is up-regulated in germ cells upon entry into meiosis. |
| Function | 3'-5' RNA helicase that plays a key role in the male and female germline by promoting transition from mitotic to meiotic divisions in stem cells. Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs that plays a role in the efficiency of RNA processing and stability. Essential for ensuring a successful progression of the meiotic program in the germline by regulating the level of m6A-containing RNAs. Acts by binding and promoting degradation of m6A-containing mRNAs: the 3'-5' RNA helicase activity is required for this process and RNA degradation may be mediated by XRN1 exoribonuclease. Required for both spermatogenesis and oogenesis. |
| Protein Name | 3'-5' Rna Helicase Ythdc2Yth Domain-Containing Protein 2Hythdc2 |
| Database Links | |
| Cellular Localisation | CytoplasmPerinuclear Region |
| Alternative Antibody Names | Anti-3'-5' Rna Helicase Ythdc2 antibodyAnti-Yth Domain-Containing Protein 2 antibodyAnti-Hythdc2 antibodyAnti-YTHDC2 antibody |
Information sourced from Uniprot.org
![Immunoprecipitation of YTHDC2 in 200 µg extracts from 293T cells using 0. 5 µg [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at 1:1000 dilution.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/177942/369971/STJ11105298_1__81591.1713135883.jpg?c=1 "Immunoprecipitation of YTHDC2 in 200 µg extracts from 293T cells using 0. 5 µg [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at 1:1000 dilution.")
