Anti-YOD1 antibody (249-348) [S5008RM] (STJ11105008)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Ubiquitin Thioesterase Otu1 (249-348) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5008RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | YOD1 |
| Gene ID | 55432 |
| Uniprot ID | OTU1_HUMAN |
| Immunogen Region | 249-348 |
| Immunogen Sequence | FGEDAGYTKRVLLIYDGIHY DPLQRNFPDPDTPPLTIFSS NDDIVLVQALELADEARRRR QFTDVNRFTLRCMVCQKGLT GQAEAREHAKETGHTNFGEV |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 249-348 of human YOD1 (NP_061036.3). |
Additional Info
| Function | Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin. May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. May recruit PLAA, UBXN6 and VCP to damaged lysosome membranes decorated with K48-linked ubiquitin chains and remove these chains allowing autophagosome formation. |
| Protein Name | Ubiquitin Thioesterase Otu1Duba-8Hiv-1-Induced Protease 7Hin-7Hshin7Otu Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-5689896 |
| Cellular Localisation | CytoplasmRecruited To Damaged Lysosomes Decorated With K48-Linked Ubiquitin Chains |
| Alternative Antibody Names | Anti-Ubiquitin Thioesterase Otu1 antibodyAnti-Duba-8 antibodyAnti-Hiv-1-Induced Protease 7 antibodyAnti-Hin-7 antibodyAnti-Hshin7 antibodyAnti-Otu Domain-Containing Protein 2 antibodyAnti-YOD1 antibodyAnti-DUBA8 antibodyAnti-HIN7 antibodyAnti-OTUD2 antibodyAnti-PRO0907 antibody |
Information sourced from Uniprot.org