| Host: | Goat |
| Applications: | WB/IHC-F/IHC-P/IF/IEM |
| Reactivity: | Transfected cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Yellow Fluorescent Protein is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immune Electron Microscopy research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 3 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC-F 1:50-1:1000IHC-P 1:50-1:1000IF 1:50-1:1000IEM 1:50-1:1000 |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Specificity: | In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes GFP and does not recognize mCherry/red fluorescent protein. |
| Immunogen: | Purified recombinant peptide produced in E. coli. |
| Background | Goat polyclonal antibody to YFP (yellow fluorescent protein). YFP is a genetic mutant of green fluorescent protein (GFP) , derived from Aequorea victoria. Its excitation/emission peaks are 514/527 nm respectively. |
Information sourced from Uniprot.org