• STJ71008 (0. 1µg/ml) staining of Human Thyroid lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ71008 (3. 8µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-XRCC4-like factor/NHEJ1 antibody (C-Term) (STJ71008)

SKU:
STJ71008-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-XRCC4-like factor/NHEJ1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-Recommended concentration, 2.5-3.8µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NHEJ1
Gene ID: 79840
Uniprot ID: NHEJ1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_079058.1
Immunogen Sequence: QRPQLSKVKRKKPR
Post Translational Modifications Phosphorylated by PRKDC at the C-terminus in response to DNA damage. Phosphorylations by PRKDC at the C-terminus of XRCC4 and NHEJ1/XLF are highly redundant and regulate ability of the XRCC4-NHEJ1/XLF subcomplex to bridge DNA. Phosphorylation does not prevent interaction with XRCC4 but disrupts ability to bridge DNA and promotes detachment from DNA.
Function DNA repair protein involved in DNA non-homologous end joining (NHEJ).required for double-strand break (DSB) repair and V(D)J recombination. Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends. Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends. May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. Associates with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors. Binds DNA in a length-dependent manner.
Protein Name Non-Homologous End-Joining Factor 1
Protein Cernunnos
Xrcc4-Like Factor
Database Links Reactome: R-HSA-5693571
Cellular Localisation Nucleus
Chromosome
Localizes To Site Of Double-Strand Breaks
Recruitment Is Dependent On Xrcc5-Xrcc6 (Ku) Heterodimer
Alternative Antibody Names Anti-Non-Homologous End-Joining Factor 1 antibody
Anti-Protein Cernunnos antibody
Anti-Xrcc4-Like Factor antibody
Anti-NHEJ1 antibody
Anti-XLF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance