• Immunohistochemistry analysis of paraffin-embedded rat lung using XRCC2 Rabbit polyclonal antibody (STJ11103888) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using XRCC2 Rabbit polyclonal antibody (STJ11103888) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using XRCC2 antibody (STJ11103888) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-XRCC2 antibody (1-83) (STJ11103888)

SKU:
STJ11103888

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Dna Repair Protein Xrcc2 (1-83) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XRCC2
Gene ID: 7516
Uniprot ID: XRCC2_HUMAN
Immunogen Region: 1-83
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-83 of human XRCC2 (NP_005422.1).
Immunogen Sequence: MCSAFHRAESGTELLARLEG RSSLKEIEPNLFADEDSPVH GDILEFHGPEGTGKTEMLYH LTARCILPKSEGGLEVEVLF IDT
Function Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Protein Name Dna Repair Protein Xrcc2
X-Ray Repair Cross-Complementing Protein 2
Database Links Reactome: R-HSA-5685942
Reactome: R-HSA-5693554
Reactome: R-HSA-5693568
Reactome: R-HSA-5693579
Reactome: R-HSA-5693616
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709603
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Alternative Antibody Names Anti-Dna Repair Protein Xrcc2 antibody
Anti-X-Ray Repair Cross-Complementing Protein 2 antibody
Anti-XRCC2 antibody

Information sourced from Uniprot.org

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