• Immunofluorescence analysis of U-2 OS cells using XK Rabbit polyclonal antibody (STJ117303) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using XK Rabbit polyclonal antibody (STJ117303) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using XK Rabbit polyclonal antibody (STJ117303) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using XK Rabbit polyclonal antibody (STJ117303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-XK antibody (59-138) (STJ117303)

SKU:
STJ117303

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-XK (59-138) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XK
Gene ID: 7504
Uniprot ID: XK_HUMAN
Immunogen Region: 59-138
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 59-138 of human XK (NP_066569.1).
Immunogen Sequence: HRDLSRDRPLVLLLHLLQLG PLFRCFEVFCIYFQSGNNEE PYVSITKKRQMPKNGLSEEI EKEVGQAEGKLITHRSAFSR
Tissue Specificity High levels in skeletal muscle, heart, brain, and pancreas.low levels in placenta, lung, liver, and kidney.
Post Translational Modifications Not glycosylated.
Function Recruits the lipid transfer protein VPS13A from lipid droplets to the endoplasmic reticulum (ER) membrane.
Protein Name Endoplasmic Reticulum Membrane Adapter Protein Xk
Kell Complex 37 Kda Component
Kx Antigen
Membrane Transport Protein Xk
Xk-Related Protein 1
Database Links Reactome: R-HSA-375276
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Endoplasmic Reticulum Membrane Adapter Protein Xk antibody
Anti-Kell Complex 37 Kda Component antibody
Anti-Kx Antigen antibody
Anti-Membrane Transport Protein Xk antibody
Anti-Xk-Related Protein 1 antibody
Anti-XK antibody
Anti-XKR1 antibody
Anti-XRG1 antibody

Information sourced from Uniprot.org

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