• Immunohistochemistry analysis of WWC1 in paraffin-embedded Human esophageal using WWC1 Rabbit polyclonal antibody (STJ11101207) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of WWC1 in paraffin-embedded Rat ovary using WWC1 Rabbit polyclonal antibody (STJ11101207) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of WWC1 in paraffin-embedded Mouse spinal cord using WWC1 Rabbit polyclonal antibody (STJ11101207) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using WWC1 Rabbit polyclonal antibody (STJ11101207) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WWC1 antibody (290-426) (STJ11101207)

SKU:
STJ11101207

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WWC1 (290-426) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WWC1
Gene ID: 23286
Uniprot ID: KIBRA_HUMAN
Immunogen Region: 290-426
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-426 of human WWC1 (NP_056053.1).
Immunogen Sequence: FGINSNNQLAEKVRLRLRYE EAKRRIANLKIQLAKLDSEA WPGVLDSERDRLILINEKEE LLKEMRFISPRKWTQGEVEQ LEMARKRLEKDLQAARDTQS KALTERLKLNSKRNQLVREL EEATRQVATLHSQLKSL
Tissue Specificity Expressed in mammary epithelial cells and breast cancer cell lines. Found in the luminal epithelium surrounding the ducts in the normal breast. In the brain, expressed in somatodendritic compartment of neurons in the cortex and hippocampus and in the cerebellum it is found in the Purkinje cells and some granule cells (at protein level). Detected in brain, heart, colon and kidney. In the kidney, expressed in glomerular podocytes, in some tubules and in the collecting duct.
Post Translational Modifications Phosphorylation at Ser-542 and Ser-931 by CDK1 in response to spindle damage stress regulates mitotic exit, these two sites are dephosphorylated by CDC14B.
Function Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ. Plays a role in cognition and memory performance. Plays an important role in regulating AMPA-selective glutamate receptors (AMPARs) trafficking underlying synaptic plasticity and learning.
Protein Name Protein Kibra
Hbeag-Binding Protein 3
Kidney And Brain Protein
Kibra
Ww Domain-Containing Protein 1
Database Links Reactome: R-HSA-2028269
Reactome: R-HSA-9013508
Cellular Localisation Cytoplasm
Perinuclear Region
Nucleus
Cell Projection
Ruffle Membrane
Colocalizes With Prkcz In The Perinuclear Region
Alternative Antibody Names Anti-Protein Kibra antibody
Anti-Hbeag-Binding Protein 3 antibody
Anti-Kidney And Brain Protein antibody
Anti-Kibra antibody
Anti-Ww Domain-Containing Protein 1 antibody
Anti-WWC1 antibody
Anti-KIAA0869 antibody

Information sourced from Uniprot.org

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