• Western blot analysis of BeWo, using WNT7A Rabbit polyclonal antibody (STJ27378) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-WNT7A antibody (135-349) (STJ27378)

SKU:
STJ27378

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT7A
Gene ID: 7476
Uniprot ID: WNT7A_HUMAN
Immunogen Region: 135-349
Specificity: A synthetic peptide corresponding to a sequence within amino acids 101-200 of human WNT7A (NP_004616.2).
Immunogen Sequence: SREAAFTYAIIAAGVAHAIT AACTQGNLSDCGCDKEKQGQ YHRDEGWKWGGCSADIRYGI GFAKVFVDAREIKQNARTLM NLHNNEAGRKILEENMKLEC KCHGVSGSCTTKTCWTTLPQ FRELGYVLKDKYNEAVHVEP VRASRNKRPTFLKIKKPLSY RKPMDTDLVYIEKSPNYCEE DPVTGSVGTQGRACNKTAPQ
Tissue Specificity Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain.
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation. Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes. Required for normal neural stem cell proliferation in the hippocampus dentate gyrus. Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation. Promotes formation of synapses via its interaction with FZD5.
Protein Name Protein Wnt-7a
Database Links Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-7a antibody
Anti-WNT7A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance