| Short Description : | Goat polyclonal antibody anti-WNT3 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications: | Pep-ELISA/WB/IHC |
| Host: | Goat |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.I |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | WNT3 |
| Gene ID: | 7473 |
| Uniprot ID: | WNT3_HUMAN |
| Accession Number: | NP_110380.1 |
| Immunogen Region: | Internal |
| Immunogen Sequence: | CGRGHNTRTEKRKEK |
| Function | Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge. Required for normal embryonic development, and especially for limb development. |
| Protein Name | Proto-Oncogene Wnt-3Proto-Oncogene Int-4 Homolog |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-3238698Reactome: R-HSA-373080 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Proto-Oncogene Wnt-3 antibodyAnti-Proto-Oncogene Int-4 Homolog antibodyAnti-WNT3 antibodyAnti-INT4 antibody |
Information sourced from Uniprot.org
