• Immunohistochemistry analysis of paraffin-embedded rat kidney using WNT2 Rabbit polyclonal antibody (STJ28427) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using WNT2 antibody (STJ28427) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WNT2 antibody (26-360) (STJ28427)

SKU:
STJ28427

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WNT2 (26-360) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT2
Gene ID: 7472
Uniprot ID: WNT2_HUMAN
Immunogen Region: 26-360
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-360 of human WNT2 (NP_003382.1).
Immunogen Sequence: SWWYMRATGGSSRVMCDNVP GLVSSQRQLCHRHPDVMRAI SQGVAEWTAECQHQFRQHRW NCNTLDRDHSLFGRVLLRSS RESAFVYAISSAGVVFAITR ACSQGEVKSCSCDPKKMGSA KDSKGIFDWGGCSDNIDYGI KFARAFVDAKERKGKDARAL MNLHNNRAGRKAVKRFLKQE CKCHGVSGSCTLRTCWLAMA DFRKTGDYLWRKYNGAIQVV MNQDGTGFTVANERFKKPT
Tissue Specificity Expressed in brain in the thalamus, in fetal and adult lung and in placenta.
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Functions as upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons.
Protein Name Protein Wnt-2
Int-1-Like Protein 1
Int-1-Related Protein
Irp
Database Links Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-2 antibody
Anti-Int-1-Like Protein 1 antibody
Anti-Int-1-Related Protein antibody
Anti-Irp antibody
Anti-WNT2 antibody
Anti-INT1L1 antibody
Anti-IRP antibody

Information sourced from Uniprot.org

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