• Immunofluorescence analysis of U2OS cells using WNK3 Rabbit polyclonal antibody (STJ117702) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using WNK3 Rabbit polyclonal antibody (STJ117702) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using WNK3 Rabbit polyclonal antibody (STJ117702) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of HAP1, using WNK3 antibody (STJ117702) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-WNK3 antibody (500-600) (STJ117702)

SKU:
STJ117702

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WNK3 (500-600) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNK3
Gene ID: 65267
Uniprot ID: WNK3_HUMAN
Immunogen Region: 500-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 500-600 of human WNK3 (NP_065973.2).
Immunogen Sequence: AGCLEERRDSQCKSMGNVFP QPQNTTLPLAPAQQTGAECE ETEVDQHVRQQLLQRKPQQH CSSVTGDNLSEAGAASVIHS DTSSQPSVAYSSNQTMGSQM V
Tissue Specificity Expressed in brain, lung, kidney, liver and pancreas, and in fetal tissues including placenta, fetal brain, lung and kidney. Very low levels of expression were also detected in fetal heart, thymus, liver and spleen. Isoform 1 is brain-specific. Isoform 3 is kidney-specific.
Post Translational Modifications Autophosphorylated at Ser-304 and Ser-308, promoting its activity. Phosphorylation at Thr-541 prevents interaction with KLHL3 and subsequent ubiquitination and degradation by the BCR(KLHL3) complex (Probable). Ubiquitinated by the BCR(KLHL2) complex, leading to its degradation. Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation.
Function Serine/threonine-protein kinase component of the WNK3-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress. WNK3 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation. The membraneless compartment concentrates WNK3 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK3-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK. Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A4/KCC1, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity. Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx.simultaneously, phosphorylation of K-Cl cotransporters SLC12A4/KCC1, SLC12A5/KCC2 and SLC12A6/KCC3 inhibits its activity, blocking ion efflux. Phosphorylates WNK4, possibly regulating the activity of SLC12A3/NCC. May also phosphorylate NEDD4L. Also acts as a scaffold protein independently of its protein kinase activity: negatively regulates cell membrane localization of various transporters and channels, such as KCNJ1 and SLC26A9. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway.
Protein Name Serine/Threonine-Protein Kinase Wnk3
Protein Kinase Lysine-Deficient 3
Protein Kinase With No Lysine 3
Database Links Reactome: R-HSA-2672351
Cellular Localisation Cytoplasm
Mediates Formation And Localizes To Cytoplasmic Membraneless Compartment In Response To Hyperosmotic Stress
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Wnk3 antibody
Anti-Protein Kinase Lysine-Deficient 3 antibody
Anti-Protein Kinase With No Lysine 3 antibody
Anti-WNK3 antibody
Anti-KIAA1566 antibody
Anti-PRKWNK3 antibody

Information sourced from Uniprot.org

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