| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-WNK3 (500-600) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WNK3 |
| Gene ID: |
65267 |
| Uniprot ID: |
WNK3_HUMAN |
| Immunogen Region: |
500-600 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 500-600 of human WNK3 (NP_065973.2). |
| Immunogen Sequence: |
AGCLEERRDSQCKSMGNVFP QPQNTTLPLAPAQQTGAECE ETEVDQHVRQQLLQRKPQQH CSSVTGDNLSEAGAASVIHS DTSSQPSVAYSSNQTMGSQM V |
| Tissue Specificity | Expressed in brain, lung, kidney, liver and pancreas, and in fetal tissues including placenta, fetal brain, lung and kidney. Very low levels of expression were also detected in fetal heart, thymus, liver and spleen. Isoform 1 is brain-specific. Isoform 3 is kidney-specific. |
| Post Translational Modifications | Autophosphorylated at Ser-304 and Ser-308, promoting its activity. Phosphorylation at Thr-541 prevents interaction with KLHL3 and subsequent ubiquitination and degradation by the BCR(KLHL3) complex (Probable). Ubiquitinated by the BCR(KLHL2) complex, leading to its degradation. Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation. |
| Function | Serine/threonine-protein kinase component of the WNK3-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress. WNK3 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation. The membraneless compartment concentrates WNK3 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK3-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK. Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A4/KCC1, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity. Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx.simultaneously, phosphorylation of K-Cl cotransporters SLC12A4/KCC1, SLC12A5/KCC2 and SLC12A6/KCC3 inhibits its activity, blocking ion efflux. Phosphorylates WNK4, possibly regulating the activity of SLC12A3/NCC. May also phosphorylate NEDD4L. Also acts as a scaffold protein independently of its protein kinase activity: negatively regulates cell membrane localization of various transporters and channels, such as KCNJ1 and SLC26A9. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. |
| Protein Name | Serine/Threonine-Protein Kinase Wnk3Protein Kinase Lysine-Deficient 3Protein Kinase With No Lysine 3 |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | CytoplasmMediates Formation And Localizes To Cytoplasmic Membraneless Compartment In Response To Hyperosmotic Stress |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Wnk3 antibodyAnti-Protein Kinase Lysine-Deficient 3 antibodyAnti-Protein Kinase With No Lysine 3 antibodyAnti-WNK3 antibodyAnti-KIAA1566 antibodyAnti-PRKWNK3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
