Western blot analysis of various lysates using WLS Rabbit polyclonal antibody (STJ11100284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-WLS antibody (43-232) (STJ11100284)

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STJ11100284
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WLS
Gene ID: 79971
Uniprot ID: WLS_HUMAN
Immunogen Region: 43-232
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 43-232 of human WLS (NP_001002292.3).
Immunogen Sequence: YMSVKCVDARKNHHKTKWFV PWGPNHCDKIRDIEEAIPRE IEANDIVFSVHIPLPHMEMS PWFQFMLFILQLDIAFKLNN QIRENAEVSMDVSLAYRDDA FAEWTEMAHERVPRKLKCTF TSPKTPEHEGRYYECDVLPF MEIGSVAHKFYLLNIRLPVN EKKKINVGIGEIKDIRLVGI HQNGGFTKVW
Post Translational Modifications N-glycosylated.
Function Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.
Protein Name Protein Wntless Homolog
Integral Membrane Protein Gpr177
Protein Evenness Interrupted Homolog
Evi
Putative Nf-Kappa-B-Activating Protein 373
Database Links Reactome: R-HSA-3238698
Cellular Localisation Golgi Apparatus Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle Membrane
Cell Membrane
Endoplasmic Reticulum Membrane
Early Endosome Membrane
Co-Localizes With The Adaptin Ap2a2 At Distinct Punctae
Alternative Antibody Names Anti-Protein Wntless Homolog antibody
Anti-Integral Membrane Protein Gpr177 antibody
Anti-Protein Evenness Interrupted Homolog antibody
Anti-Evi antibody
Anti-Putative Nf-Kappa-B-Activating Protein 373 antibody
Anti-WLS antibody
Anti-C1orf139 antibody
Anti-GPR177 antibody
Anti-UNQ85 antibody
Anti-PRO18667 antibody

Information sourced from Uniprot.org

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