• STJ70963 (1µg/ml) staining of Human Spleen (A) , Kidney (B) and Testis (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Wilms tumor 1/WT1 antibody (N-Term) (STJ70963)

SKU:
STJ70963-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Wilms tumor 1/WT1 (N-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: WT1
Gene ID: 7490
Uniprot ID: WT1_HUMAN
Immunogen Region: N-Term
Accession Number: NP_000369.3; NP_077742.2; NP_077743.2; NP_077744.3
Specificity: This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).
Immunogen Sequence: QDPASTCVPEPASQH
Function Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
Protein Name Wilms Tumor Protein
Wt33
Database Links Reactome: R-HSA-9690406
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Isoforms Lacking The Kts Motif Have A Diffuse Nuclear Location
Shuttles Between Nucleus And Cytoplasm
Isoform 1: Nucleus Speckle
Isoform 4: Nucleus
Nucleoplasm
Alternative Antibody Names Anti-Wilms Tumor Protein antibody
Anti-Wt33 antibody
Anti-WT1 antibody

Information sourced from Uniprot.org

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