Host: | Goat |
Applications: | Pep-ELISA/WB |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Goat polyclonal antibody anti-Wilms tumor 1/WT1 (N-Term) is suitable for use in ELISA and Western Blot research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3kDa according to NP_000369.3). Recommended concentration: 1-3µg/ml. |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | WT1 |
Gene ID: | 7490 |
Uniprot ID: | WT1_HUMAN |
Immunogen Region: | N-Term |
Accession Number: | NP_000369.3; NP_077742.2; NP_077743.2; NP_077744.3 |
Specificity: | This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3). |
Immunogen Sequence: | QDPASTCVPEPASQH |
Function | Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. |
Protein Name | Wilms Tumor ProteinWt33 |
Database Links | Reactome: R-HSA-9690406Reactome: R-HSA-9831926 |
Cellular Localisation | NucleusNucleolusCytoplasmIsoforms Lacking The Kts Motif Have A Diffuse Nuclear LocationShuttles Between Nucleus And CytoplasmIsoform 1: Nucleus SpeckleIsoform 4: NucleusNucleoplasm |
Alternative Antibody Names | Anti-Wilms Tumor Protein antibodyAnti-Wt33 antibodyAnti-WT1 antibody |
Information sourced from Uniprot.org