| Host: | Rabbit |
| Applications: | ELISA/IP/WB |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-WEE2 (60-110) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5-1.5 µg/µl |
| Dilution Range: | WB: 1:2, 500-1:5, 000 ELISA: 1:50, 000 IP: 1:200 IHC: 1:50-1:200 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Wee2 |
| Gene ID: | 381759 |
| Uniprot ID: | WEE2_MOUSE |
| Immunogen Region: | 60-110 |
| Immunogen: | Synthetic peptide taken within amino acid region 60-110 on mouse Wee1-like protein kinase isoform 2. |
| Post Translational Modifications | Phosphorylated by PKA at Ser-15 in vitro, leading to activate kinase activity. Phosphorylation at Ser-15 by CaMK2, leading to increase its activity and promote metaphase II exit during egg activation. |
| Function | Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation. |
| Protein Name | Wee1-Like Protein Kinase 2 Wee1-Like Protein Kinase 1b Wee1b Kinase Mwee1b |
| Cellular Localisation | Cytoplasm Nucleus Localizes Mainly In The Nucleus Exported From The Nucleus To The Cytoplasm Before Germinal Vesicle Breakdown (Gvbd) Allowing Meiosis Resumption |
| Alternative Antibody Names | Anti-Wee1-Like Protein Kinase 2 antibody Anti-Wee1-Like Protein Kinase 1b antibody Anti-Wee1b Kinase antibody Anti-Mwee1b antibody Anti-Wee2 antibody Anti-Wee1b antibody |
Information sourced from Uniprot.org