Anti-Wee1 antibody (500-550) {FITC} (STJ503523)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationFITC
IsotypeIgG
ImmunogenSynthetic peptide taken within amino acid region 500-550 on human Wee1-like protein kinase isoform 1.
General Information
| Short Description | Rabbit polyclonal antibody anti-Wee1 (500-550) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | FITC |
| Concentration | 0.5-1.5 µg/µl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:2, 500-1:5, 000ELISA: 1:50, 000IP: 1:200 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | WEE1 |
| Gene ID | 7465 |
| Uniprot ID | WEE1_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 500-550 on human Wee1-like protein kinase isoform 1. |
| Immunogen Region | 500-550 |
Additional Info
| Function | Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. |
| Protein Name | Wee1-Like Protein KinaseWee1huWee1a Kinase |
| Database Links | Reactome: R-HSA-156711Reactome: R-HSA-69202Reactome: R-HSA-69273Reactome: R-HSA-69478Reactome: R-HSA-69656Reactome: R-HSA-75035Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Wee1-Like Protein Kinase antibodyAnti-Wee1hu antibodyAnti-Wee1a Kinase antibodyAnti-WEE1 antibody |
Information sourced from Uniprot.org