Anti-Wee1 antibody (500-550) {Biotin} (STJ503522)

SKU:
STJ503522-100

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Host: Rabbit
Applications: ELISA/IHC/IP/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Wee1 (500-550) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.5-1.5 µg/µl
Dilution Range: WB: 1:2, 500-1:5, 000
ELISA: 1:50, 000
IP: 1:200
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: WEE1
Gene ID: 7465
Uniprot ID: WEE1_HUMAN
Immunogen Region: 500-550
Immunogen: Synthetic peptide taken within amino acid region 500-550 on human Wee1-like protein kinase isoform 1.
Function Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Protein Name Wee1-Like Protein Kinase
Wee1hu
Wee1a Kinase
Database Links Reactome: R-HSA-156711
Reactome: R-HSA-69202
Reactome: R-HSA-69273
Reactome: R-HSA-69478
Reactome: R-HSA-69656
Reactome: R-HSA-75035
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Wee1-Like Protein Kinase antibody
Anti-Wee1hu antibody
Anti-Wee1a Kinase antibody
Anti-WEE1 antibody

Information sourced from Uniprot.org

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