• Immunohistochemistry analysis of paraffin-embedded mouse lung using WEE1 Rabbit monoclonal antibody (STJ11104785) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-WEE1 antibody (1-100) [S4785RM] (STJ11104785)

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STJ11104785

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Host: Rabbit
Applications: IHC
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Wee1-Like Protein Kinase (1-100) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4785RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WEE1
Gene ID: 7465
Uniprot ID: WEE1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human WEE1 (NP_003381.1).
Immunogen Sequence: MSFLSRQQPPPPRRAGAACT LRQKLIFSPCSDCEEEEEEE EEEGSGHSTGEDSAFQEPDS PLPPARSPTEPGPERRRSPG PAPGSPGELEEDLLLPGACP
Post Translational Modifications Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase. Dephosphorylated at Thr-239 by CTDP1. Dephosphorylated at Ser-53 and Ser-123 by the serine/threonine-protein phosphatase 2A preventing its ubiquitin-mediated degradation. Ubiquitinated and degraded at the onset of G2/M phase.
Function Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Protein Name Wee1-Like Protein Kinase
Wee1hu
Wee1a Kinase
Database Links Reactome: R-HSA-156711
Reactome: R-HSA-69202
Reactome: R-HSA-69273
Reactome: R-HSA-69478
Reactome: R-HSA-69656
Reactome: R-HSA-75035
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Wee1-Like Protein Kinase antibody
Anti-Wee1hu antibody
Anti-Wee1a Kinase antibody
Anti-WEE1 antibody

Information sourced from Uniprot.org

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