| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-WDR48 (450-677) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WDR48 |
| Gene ID: |
57599 |
| Uniprot ID: |
WDR48_HUMAN |
| Immunogen Region: |
450-677 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 450-677 of human WDR48 (NP_065890.1). |
| Immunogen Sequence: |
FSSPDGSDPKLNLGGLLLQA LLEYWPRTHVNPMDEEENEV NHVNGEQENRVQKGNGYFQV PPHTPVIFGEAGGRTLFRLL CRDSGGETESMLLNETVPQW VIDITVDKNMPKFNKIPFYL QPHASSGAKTLKKDRLSASD MLQVRKVMEHVYEKIINLDN ESQTTSSSNNEKPGEQEKEE DIAVLAEEKIELLCQDQVLD PNMDLRTVKHFIWKSGGDLT LHYRQKST |
| Tissue Specificity | Ubiquitous. |
| Function | Regulator of deubiquitinating complexes, which acts as a strong activator of USP1, USP12 and USP46. Enhances the USP1-mediated deubiquitination of FANCD2.USP1 being almost inactive by itself. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. Also activates deubiquitinating activity of complexes containing USP12. In complex with USP12, acts as a potential tumor suppressor by positively regulating PHLPP1 stability. Docks at the distal end of the USP12 fingers domain and induces a cascade of structural changes leading to the activation of the enzyme. Together with RAD51AP1, promotes DNA repair by stimulating RAD51-mediated homologous recombination. Binds single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). DNA-binding is required both for USP1-mediated deubiquitination of FANCD2 and stimulation of RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during these processes. Together with ATAD5 and by regulating USP1 activity, has a role in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Together with ATAD5, has a role in recruiting RAD51 to stalled forks during replication stress. (Microbial infection) In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1. |
| Protein Name | Wd Repeat-Containing Protein 48Usp1-Associated Factor 1Wd Repeat Endosomal ProteinP80 |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-5689880Reactome: R-HSA-6783310Reactome: R-HSA-9673766 |
| Cellular Localisation | NucleusCytoplasmLysosomeLate EndosomeMainly In Cytoplasmic CompartmentsIn Case Of Infection By Papillomavirus Hpv11Translocates To The Nucleus Via Its Interaction With Papillomavirus Hpv11 |
| Alternative Antibody Names | Anti-Wd Repeat-Containing Protein 48 antibodyAnti-Usp1-Associated Factor 1 antibodyAnti-Wd Repeat Endosomal Protein antibodyAnti-P80 antibodyAnti-WDR48 antibodyAnti-KIAA1449 antibodyAnti-UAF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
