• Immunofluorescence analysis of U-2 OS cells using WDHD1 Rabbit polyclonal antibody (STJ117591) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of WDHD1 in paraffin-embedded Human esophageal cancer using WDHD1 Rabbit polyclonal antibody (STJ117591) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using WDHD1 Rabbit polyclonal antibody (STJ117591) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WDHD1 antibody (830-1129) (STJ117591)

SKU:
STJ117591

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WDHD1 (830-1129) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WDHD1
Gene ID: 11169
Uniprot ID: WDHD1_HUMAN
Immunogen Region: 830-1129
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 830-1129 of human WDHD1 (NP_009017.1).
Immunogen Sequence: EEEEEEDFRKKLNAGYSNTA TEWSQPRFRNQVEEDAEDSG EADDEEKPEIHKPGQNSFSK STNSSDVSAKSGAVTFSSQG RVNPFKVSASSKEPAMSMNS ARSTNILDNMGKSSKKSTAL SRTTNNEKSPIIKPLIPKPK PKQASAASYFQKRNSQTNKT EEVKEENLKNVLSETPAICP PQNTENQRPKTGFQMWLEEN RSNILSDNPDFSDEADIIKE GMIRFRVLSTEERKVWANK
Function Core replisome component that acts as a replication initiation factor. Binds directly to the CMG complex and functions as a hub to recruit additional proteins to the replication fork.
Protein Name Wd Repeat And Hmg-Box Dna-Binding Protein 1
Acidic Nucleoplasmic Dna-Binding Protein 1
And-1
Cellular Localisation Nucleus
Nucleoplasm
Alternative Antibody Names Anti-Wd Repeat And Hmg-Box Dna-Binding Protein 1 antibody
Anti-Acidic Nucleoplasmic Dna-Binding Protein 1 antibody
Anti-And-1 antibody
Anti-WDHD1 antibody
Anti-AND1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance