| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-N-WASP (N-Term) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WASL |
| Gene ID: |
8976 |
| Uniprot ID: |
WASL_HUMAN |
| Immunogen Region: |
N-Term |
| Specificity: |
This antibody detects a 65 kDa* protein corresponding to the molecular mass of N-WASP on SDS-PAGE immunoblots of neonatal rat brain lysate. It is also detects 65 kDa* proteins in A431, human endothelial, and SKN-SH cells. It does not recognize the 63 |
| Immunogen: |
N-WASP synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human N-WASP. This N-WASP peptide sequence is 100% homologous to rat and mouse N-WASP, and has low homology to the corresponding region in the |
| Function | Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis. Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear. |
| Protein Name | Actin Nucleation-Promoting Factor WaslNeural Wiskott-Aldrich Syndrome ProteinN-Wasp |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-203641Reactome: R-HSA-373753Reactome: R-HSA-3928662Reactome: R-HSA-418885Reactome: R-HSA-5663213Reactome: R-HSA-8856828Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013406Reactome: R-HSA-9013409Reactome: R-HSA-9013424Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCytoskeletonNucleusPreferentially Localized In The Cytoplasm When Phosphorylated And In The Nucleus When UnphosphorylatedExported From The Nucleus By An Nuclear Export Signal (Nes)-Dependent Mechanism To The Cytoplasm |
| Alternative Antibody Names | Anti-Actin Nucleation-Promoting Factor Wasl antibodyAnti-Neural Wiskott-Aldrich Syndrome Protein antibodyAnti-N-Wasp antibodyAnti-WASL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
