| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-WASH complex subunit 1 (163-213 aa) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WASHC1 |
| Gene ID: |
100287171 |
| Uniprot ID: |
WASH1_HUMAN |
| Immunogen Region: |
163-213 aa |
| Specificity: |
This antibody detects endogenous levels of WASH1 at Human/Mouse/Rat |
| Immunogen: |
Synthesized peptide derived from the human WASH1 at the amino acid range 163-213 |
| Function | Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Involved in endocytic trafficking of EGF. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL.proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity. |
| Protein Name | Wash Complex Subunit 1Cxyorf1-Like Protein On Chromosome 9Protein Fam39eWas Protein Family Homolog 1 |
| Cellular Localisation | Early Endosome MembraneRecycling Endosome MembraneLate EndosomeCytoplasmic VesicleAutophagosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleLocalization To The Endosome Membrane Is Mediated Via Its Interaction With Washc2Localizes To Mmp14-Positive Late Endosomes And Transiently To InvadipodiaLocalized To Salmonella Typhimurium Entry Sites |
| Alternative Antibody Names | Anti-Wash Complex Subunit 1 antibodyAnti-Cxyorf1-Like Protein On Chromosome 9 antibodyAnti-Protein Fam39e antibodyAnti-Was Protein Family Homolog 1 antibodyAnti-WASHC1 antibodyAnti-FAM39E antibodyAnti-WASH1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
