Anti-VRK3 antibody (Internal) (STJ96258)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the Internal region of human VRK3.
General Information
| Short Description | Rabbit polyclonal anti-Inactive serine/threonine-protein kinase VRK3 (Internal) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VRK3 |
| Gene ID | 51231 |
| Uniprot ID | VRK3_HUMAN |
| Immunogen | Synthesized peptide derived from the Internal region of human VRK3. |
| Immunogen Region | Internal |
| Specificity | VRK3 Polyclonal Antibody detects endogenous levels of VRK3 protein. |
Additional Info
| Function | Plays a role in the regulation of the cell cycle by phosphorylating the nuclear envelope protein barrier-to-autointegration factor/BAF that is required for disassembly and reassembly, respectively, of the nuclear envelope during mitosis. Under normal physiological conditions, negatively regulates ERK activity along with VHR/DUSP3 phosphatase in the nucleus, causing timely and transient action of ERK. Stress conditions activate CDK5 which phosphorylates VRK3 to increase VHR phosphatase activity and suppress prolonged ERK activation that causes cell death. For example, upon glutamate induction, promotes nuclear localization of HSP70/HSPA1A to inhibit ERK activation via VHR/DUSP3 phosphatase. |
| Protein Name | Serine/Threonine-Protein Kinase Vrk3Vaccinia-Related Kinase 3 |
| Database Links | Reactome: R-HSA-202670 |
| Cellular Localisation | NucleusCytoplasmUnder Oxidative StressMigrates From The Nucleus To The Cytoplasm |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Vrk3 antibodyAnti-Vaccinia-Related Kinase 3 antibodyAnti-VRK3 antibody |
Information sourced from Uniprot.org