| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-VPS4A (1-120) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
VPS4A |
| Gene ID: |
27183 |
| Uniprot ID: |
VPS4A_HUMAN |
| Immunogen Region: |
1-120 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human VPS4A (NP_037377.1). |
| Immunogen Sequence: |
MTTSTLQKAIDLVTKATEED KAKNYEEALRLYQHAVEYFL HAIKYEAHSDKAKESIRAKC VQYLDRAEKLKDYLRSKEKH GKKPVKENQSEGKGSDSDSE GDNPEKKKLQEQLMGAVVME |
| Tissue Specificity | Ubiquitously expressed. |
| Function | Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. It is required for proper accomplishment of various processes including the regulation of endosome size, primary cilium organization, mitotic spindle organization, chromosome segregation, and nuclear envelope sealing and spindle disassembly during anaphase. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan. Critical for normal erythroblast cytokinesis and correct erythropoiesis. (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 4aProtein Skd2Vps4-1Hvps4 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-917729Reactome: R-HSA-9610379Reactome: R-HSA-9668328 |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinMidbodyCytoplasmCytoskeletonSpindleMembrane-Associated In The Prevacuolar Endosomal CompartmentLocalizes To The Midbody Of Dividing CellsInteraction With Zfyve19/Anchr Mediates Retention At MidbodyLocalized In Two Distinct Rings On Either Side Of The Flemming Body |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 4a antibodyAnti-Protein Skd2 antibodyAnti-Vps4-1 antibodyAnti-Hvps4 antibodyAnti-VPS4A antibodyAnti-VPS4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
